Successive grinding and sieving as a new tool to ...
In brief, 0.1 ml diluted and filtered hydromethanolic extract was added to 2.4 ml of distilled water and 0.15 ml of 5% Na 2 NO 2 (w/v). After 6 min, 0.3 ml of 10% aluminum chloride (AlCl 3 ·6H 2 O) (w/v) was added. The mixture was kept at room temperature for 5 min, and 1 ml NaOH (1 M) was added.