RESEARCH Open Access High frequency microcloning of Aloe …
In vivo mother plant and field grown two years old ex vitro micropropagated plants were used for cytological analysis. Total 25 root tips of mother plant as well as 125 root tips of 25 randomly selected micropropagated plants were excised, washed with tap water, and pre-treated with a saturated solution of Þ-dichlorobenzene for 4 h at 16-18°C.